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| While at Monsanto Potentiation of Bacillus thuringiensis Insecticidal Activity by Serine Protease Inhibitors Insect Tolerant Transgenic Tomato Plants Field Performance of Transgenic Tomato Plants Expressing the Bacillus Thuringiensis Var. Kurstaki Insect Control Protein Characterization of the Coleopteran−Specific Protein Gene of Bacillus thuringiensis Var. tenebrionis PATENTS Strain of bacillus for controlling plant diseases and corn rootworm Compositions and methods for controlling plant pests Antibiotic-producing strain of bacillus and methods for controlling plant diseases Compositions comprising EMU oil or active fractions thereof and methods of using these compositions as insect repellents US Patent 5801196 - Methods of using emu oil and active fractions thereof as an insect repellentBiological control of plant fungal infections | Several serine protease inhibitors enhanced the insecticidal activity of the insect control proteins from Bacillus thuringiensis var. kurstaki, var. tenebrionis, and var. israelensis against their target insects, tobacco budworm and other lepidopterans, Colorado potato beetle, and mosquito, respectively. B. thuringiensis protein concentrations, at levels causing minimal insect mortality (10-20%), were mixed with purified protease inhibitors and assayed against the appropriate target insects. The presence of extremely low levels (4 FM) of protease inhibitors enhanced the insecticidal activity of B. thuringiensis var. kurstaki by 2-20-fold. Protease inhibitors also potentiated the insect growth inhibitory activity of sublethal B. thuringiensis var. kurstaki protein concentrations. Genetically improved tobacco plants expressing a protease inhibitor fused to a truncated B. thuringiensis insect control protein showed levels of activity enhancement similar to those seen with purified protease inhibitors. http://pubs.acs.org/cgi-bin/abstract.cgi/jafcau/1990/38/i04/f-pdf/f_jf00094a051.pdf?sessid=6006l3 http://www.nature.com/nbt/journal/v5/n8/abs/nbt0887-807.html Bio/Technology 5, 807 - 813 (1987) doi:10.1038/nbt0887-807 Insect Tolerant Transgenic Tomato Plants Present address: Research Institute of Scripps Clinic, La Jolla, CA. Present address: Plant Gene Expression Center, USDA-ARS, Albany, CA. The structure of an insect control protein gene from Bacillus thuringiensis var. kurstaki HD−1 was determined, and truncated forms of the gene that express a functional insecticidal protein were generated. Two of these truncated genes were incorporated into a plant expression vector for Agrobacterium−mediated transformation. Transgenic tomato plants containing the chimeric genes express the insect control protein gene. Such expression confers tolerance to lepidopteran larvae on the transgenic tomato plants and their progeny. These engineered tomato plants represent a significant step to increased selectivity, specificity and efficiency in insect control. Bio/Technologyhttp://www.nature.com/nbt/journal/v7/n12/abs/nbt1289-1265.html Field Performance of Transgenic Tomato Plants Expressing the Bacillus Thuringiensis Var. Kurstaki Insect Control ProteinTransgenic tomato plants expressing a lepidopteran-specific insect control protein from Bacillus thuringiensis var. kurstaki HD-1 (B.t.k.) were field tested in 1987 and 1988 to evaluate their efficacy under normal field conditions. When infested with eggs of the tobacco hornworm (Manduca sexta), the transgenic plants showed only very limited feeding damage on the leaves near the inoculated sites soon after the larvae hatched, and sustained no further damage. In contrast, nontransgenic control plants suffered heavy feeding damage and were almost completely defoliated within two weeks. Significant control of tomato fruitworm (Heliothis zea) and tomato pinworm (Keiferia lycopersicella) was also observed. These results demonstrate that transgenic tomato plants containing a gene encoding a B.t.k. protein can provide control of lepidopteran insect pests under field conditions. Bio/Technology 6, 61 - 66 (1988) doi:10.1038/nbt0188-61 Characterization of the Coleopteran−Specific Protein Gene of Bacillus thuringiensis Var. tenebrionis Sylvia A. McPherson1, Frederick J. Perlak1, *, Roy L. Fuchs1, Pamela G. Marrone1, Paul B. Lavrik1 & David A. Fischhoff1 Monsanto Co., 700 Chesterfield Village Parkway, St. Louis, MO 63198.
Strain of bacillus for controlling plant diseases and corn rootworm
Document Type and Number:
United States Patent 6291426
Abstract:
The present invention relates to a novel antibiotic-producing and
metabolite-producing Bacillus subtilis strain that exhibits
insecticidal, antifungal and antibacterial activity. The supernatant of
this novel strain contains effective insecticidal, antifungal and
antibacterial agents. Also included in the invention is a solvent
extractable, small molecular weight (<10,000 daltons) corn
rootworm-active metabolite produced in the supernatant. Also included
in the invention are methods of protecting or treating plants from
fungal and bacterial infections and corn rootworm infestations
comprising the step of applying to the plant an effective amount of the
antibiotic/metabolite-producing novel Bacillus subtilis strain, the
antibiotic/metabolite produced by the novel Bacillus subtilis strain or
a combination thereof, optionally further comprising another
antibiotic-producing bacterial strain and/or a chemical pesticide. The
invention also includes methods of preventing or treating fungal and
bacterial infections using whole broth cultures or supernatants
obtained from cultures of the novel Bacillus subtilis strain alone or
in combination with chemical pesticides and/or other biocontrol agents.
The invention also includes novel antifungal and antibacterial
compounds designated agrastatins and a novel combination comprising an
A-type iturin, a plipastatin, a surfactin and an agrastatin. Methods of
treating or protecting plants from fungal and bacterial infections and
corn rootworm infestations comprising administering the novel
agrastatins and the novel combination comprising an A-type iturin, a
plipastatin, a surfactin and an agrastatin are provided.
Application Number: 09/312314 Publication Date: 09/18/2001
Document Type and Number:
United States Patent 6638910
Abstract:
The present invention relates to a novel antibiotic-producing and metabolite-producing
Application Number: 09/994234
Publication Date: 10/28/2003
Filing Date: 11/27/2001
Document Type and Number:
United States Patent 5753222
Abstract:
The present invention relates to a novel strain antibiotic-producing
Bacillus subtilis which is effective against plant fungal and bacterial
infections. The invention also includes methods of protecting plants
from fungal and bacterial infections comprising the step of applying to
the plant an effective amount of the antibiotic-producing Bacillus
subtilis, the antibiotic produced by the antibiotic-producing Bacillus
subtilis or a combination thereof, optionally further comprising
another antibiotic-producing bacterial strain and/or a chemical
pesticide. The invention includes methods of treating fungal and
bacterial infections using whole broth cultures or supernatants
obtained from cultures of antibiotic-producing Bacillus subtilis
cultures. The invention also includes treating plant infections using a
combination of whole broth and/or supernatant obtained from the
antibiotic-producing strain of Bacillus subtilis optionally in
combination with chemical pesticides. Also provided is the novel
bacterial strain and the antibiotic produced by the novel strain.
Application Number: 08/746893
Publication Date: 05/19/1998
Filing Date: 11/18/1996
Compositions comprising EMU oil or active fractions thereof and methods of using these compositions as insect repellents
Document Type and Number:
United States Patent 5929113
Abstract:
This invention provides a method for repelling biting insects such as
mosquitoes, ticks, and biting flies by topically applying to the skin
of a subject emu oil, fractions of emu oil obtained by flash
chromatography or a composition comprising emu oil or active fractions
thereof and at least one other insect repellent such as citronella,
DEET or saponin. Also provided are compositions for repelling biting
insects comprising diluted fractions of emu oil, and compositions
comprising emu oil or active fractions thereof and at least one other
insect repellent such as citronella, DEET or saponin.
Inventors:
Manker, Denise C. (Davis, CA)
Marrone, Pamela Gail (Davis, CA) Judd, Stephen (Davis, CA) Application Number:08/876268 Publication Date:07/27/1999
Filing Date:06/16/1997
Document Type and Number:
United States Patent 6004774
Abstract:
The present invention is directed to a unique strain of Bacillus
subtilis for inhibiting growth of plant pathogenic fungi and bacteria
and methods for treating or protecting plants from fingal and bacterial
infections.
Inventors:
Marrone, Pamela Gail (Davis, CA)
Heins, Sherry D. (Davis, CA) Manker, Denise C. (Davis, CA) Jimenez, Desmond R. (Woodland, CA) Bestwick, Richard K. (Portland, OR) Vandemark, George J. (Prosser, WA) Application Number:09/199871 Publication Date:12/21/1999
Filing Date:11/25/1998
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